Support for Extracellular Vesicles
EVs are an important emerging class of biomarkers in early cancer detection. A number of high profile studies showcase the meaningful advances being made in this exciting area of clinical science.
Snapshot: Extracellullar Vesicles
Cell – 2020
Cocozza F, Grisard E, et al.
Extracellular vesicles (EVs) are structures released into their environment by all cells. They are delimited by a lipid-bilayer and contain components from the cells that release them. EVs have been identified as a means, for the secreting cell, of disposing harmful or useless intracellular components but also as important mediators of communication with other cells.
Modeling EV kinetics for use in early cancer detection
Advanced Biosystems – 2020
Ferguson S, Weissleder R
Tumor-derived extracellular vesicles represent promising biomarkers for monitoring cancers. It is predicted that emerging single EV methods will allow blood-based detection of cancers of <1mm in humans.
Profiling surface proteins on individual exosomes using a proximity barcoding assay
Nature Communications – 2019
Wu D, Yan J, et al.
Exosomes from different sources are characterized by the presence of specific combinations of surface proteins and their abundance, allowing exosomes to be separately quantified in mixed samples to serve as markers for tissue-specific engagement in disease.
Exploiting the message from cancer: The diagnostic value of EVs for clinical applications
Experimental and Mol. Medicine – 2019
Kosaka N, Kogure A, et al.
Extracellular vesicles (EVs), which contain various proteins, including membrane-bound proteins, and RNAs, including mRNA and long/short noncoding RNAs, have emerged as ideal targets for liquid biopsy.
Characterization of single microvesicles in plasma from glioblastoma patients
Neuro-Oncology – 2019
Fraser K, Jo A, et al.
Results indicate that single microvesicle analysis is likely necessary to identify rare tumoral microvesicle populations, and the single vesicle analytical technique used here can be applied to both microvesicle and exosome fractions without the need for their separation from each other. These studies form the basis for using single EV analyses for cancer diagnostics.
Extracellular vesicles in cancer – implications for future improvements in cancer care
Nature Reviews/Clin. Oncology – 2018
Xu R, Rai A, et al.
EVs carry molecules such as oncoproteins and oncopeptides, RNA, lipids, and DNA fragments from donor to recipient cells, initiating profound phenotypic changes in the tumor microenvironment. Emerging evidence suggests that EVs have crucial roles in cancer development, including premetastatic niche formation and metastasis.
Multiplexed profiling of single extracellular vesicles
ACS Nano – 2018
Lee K, Fraser K, et al.
The relative abundance and composition of EV proteins serves as a fingerprint that indicates their cellular origin. Importantly, single EV analyses will enable molecular identification of tumor-derived EV even in a vast biological background of host cell-derived EVs. We believe that the platform studied will be a useful analytical tool for studying different types of extracellular vesicles across different cell types at the single-particle level.
New technologies for analysis of extracellular vesicles
Chemical Reviews – 2018
Shao H, Im H, et al.
EVs are now increasingly recognized as important vehicles of intercellular communication and circulating biomarkers for disease diagnoses and prognosis. Recent advances in the field are expected to have far-reaching impact in both basic and translational studies.